Search results for "Dihydroxyacetone phosphate"
showing 3 items of 3 documents
Copurification of dihydroxyacetone-phosphate acyl-transferase and other peroxisomal proteins from liver of fenofibrate-treated rats.
1997
Dihydroxyacetone-phosphate acyl-transferase (DHAP-AT), a peroxisomal membrane-bound enzyme that catalyzes the first step of ether-glycerolipid synthesis, was purified from liver of rats treated with fenofibrate, a peroxisome proliferator. The protocol first included isolation of peroxisomes, their purification through a discontinuous gradient and solubilization of membranes in CHAPS. DHAP-AT was further purified by four chromatographic steps, namely low-pressure size-exclusion, cation-exchange, hydroxylapatite and chromatofocusing. The chromatofocusing step led to a 4000-fold increase in the specific activity of DHAP-AT with respect to the liver homogenate with a yield of about 0.2%. Trypsi…
In situ hybridization of dihydroxyacetone phosphate acyltransferase, the regulating enzyme involved in plasmalogen biosynthesis
2005
International audience; In situ hybridization can be carried out using different methods. The experimenter has to choose various parameters: the type of tissue fixation, the time of incubation, and the duration of the exposure time. All these parameters are determinant for the sensitivity and the resolution of this technique. This publication of technical aspects described different experiments performed for in situ hybridization on liver tissue. We may conclude on the parameters to optimize each step of the hybridization procedure. Moreover, this technique could be transposed to the brain and applied to little structures with a light expression of DHAP-AT.
Die α-Glycerophosphat-Oxydation des Heuschreckenbrustmuskels (Locusta migratoria)
1956
The conditions were studied for the glycerophosphate oxidation by homogenate from locust flight muscle, and the O2-consumption was measured. Maximal oxidation rates were found with 0.087m glycerophosphate, 8 × 10−6m cytochromec, 7 × 10−6m DPN and pH 7.5. The production of dihydroxyacetone phosphate is followed by further oxidation steps, as could be shown by estimation of the different fractions of acid-soluble phosphate. Comparative studies were made on different insects and vertebrates. The rate of succinate oxidation by insect muscle was found to be ten times higher than that of vertebrate muscle. The relation of glycerophosphate oxidation to succinate oxidation is quite different in ins…